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dapi nuclear roi  (Oxford Instruments)


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    Structured Review

    Oxford Instruments dapi nuclear roi
    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), <t>DAPI</t> (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).
    Dapi Nuclear Roi, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44474 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+nuclear+roi/Imaris/bio_rxiv__2025__09__13__676046-93-11-7
    Average 99 stars, based on 44474 article reviews
    dapi nuclear roi - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease"

    Article Title: Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease

    Journal: bioRxiv

    doi: 10.1101/2025.09.13.676046

    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).
    Figure Legend Snippet: Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).

    Techniques Used: Derivative Assay, Staining, Mutagenesis

    Related Articles

    Derivative Assay:

    Article Title: Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease
    Article Snippet: analysis was performed on 5 neurons per genotype using Bitplane Imaris software (Oxford Instruments). .. A surface object workflow was used in Imaris to define the DAPI+ nuclear ROI. .. Neuronal nuclei were identified by the presence of MAP2 cytoplasmic staining.

    Staining:

    Article Title: Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease
    Article Snippet: analysis was performed on 5 neurons per genotype using Bitplane Imaris software (Oxford Instruments). .. A surface object workflow was used in Imaris to define the DAPI+ nuclear ROI. .. Neuronal nuclei were identified by the presence of MAP2 cytoplasmic staining.

    Mutagenesis:

    Article Title: Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease
    Article Snippet: analysis was performed on 5 neurons per genotype using Bitplane Imaris software (Oxford Instruments). .. A surface object workflow was used in Imaris to define the DAPI+ nuclear ROI. .. Neuronal nuclei were identified by the presence of MAP2 cytoplasmic staining.



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    dapi nuclear roi  (Oxford Instruments)
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    Oxford Instruments dapi nuclear roi
    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), <t>DAPI</t> (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).
    Dapi Nuclear Roi, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+nuclear+roi/Imaris/bio_rxiv__2025__09__13__676046-93-11-7
    Average 99 stars, based on 1 article reviews
    dapi nuclear roi - by Bioz Stars, 2026-11
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).

    Journal: bioRxiv

    Article Title: Kat5 cKO Biological Domain Signatures Align with Human Alzheimer’s Disease

    doi: 10.1101/2025.09.13.676046

    Figure Lengend Snippet: Subcellular localization of KAT5 in human iPSC-derived WT and FAD neurons. Induced-pluripotent stem cells (iPSCs) from a WT CViia2 strain, APP Swedish (APPswed) and APP duplication mutants (APPdup, a model of trisomy 21) were differentiated into glutamatergic neurons and stained for KAT5 (green), DAPI (blue) to show nuclear localization and MAP2 (red) to show cytosolic cytoskeletal structure. The wild-type cells demonstrate ubiquitous localization (A) with fluorescent overlap with the DAPI nuclear signal. All explored FAD mutant lines (B and C) and APP duplication lines (D) show specific nuclear exclusion of KAT5. The WT CViia2 neurons treated with gamma-secretase inhibitor DAPT demonstrate nuclear exclusion of KAT5 (E). The statistical significance was analyzed as described in methods section across cells per culture group, with the average for each cell-type shown as a single dot in the plots on the right (F and G). The WT cells are statistically significantly different in nuclear signal intensity than either the APPSWE heterozygous cells (p<0.01), the APP duplication (p<0.05), and PSEN2 N141I heterozygous mutants (p<0.01). The gamma-secretase inhibitor treated cells approached statistical significance (p<0.09).

    Article Snippet: A surface object workflow was used in Imaris to define the DAPI+ nuclear ROI.

    Techniques: Derivative Assay, Staining, Mutagenesis